Why 3D cell culture?

Today, there is increasing awareness of the drawbacks of 2D cell culture to predict the complex behavior of a biological system of many different interacting cell types and to reproduce the anatomy or physiology of a tissue for informative or useful study.

3D cell culture models are a more accurate representation of the natural environment experienced by the cells in the living organism, which allows for intercellular interactions with more realistic biochemical and physiological responses. Cells behave and respond more like they would in vivo to internal and external stimuli, such as changes in temperature, pH, nutrient absorption, transport, and differentiation. Therefore, scientists are shifting their focus from 2D to 3D cell culture in the fields of drug screening, tissue engineering, preclinical study, cell therapy and basic cell biological study.

According to PubMed, the published number about 3D cell culture have grown dramatically in past 20 years.

As 3D culture systems become more mature and relevant to human and animal physiology, the ability to design and develop co-cultures becomes possible as does the ability to integrate stem cells. The primary objectives for developing 3D cell culture systems vary widely and range from engineering tissues for clinical delivery through to the development of models for drug screening.

How does VitroGel 3D bridge in vitro and in vivo studies?

Mixing VitroGel 3D solution and the cell culture media at proper ratio (We recommend VitroGel: cell media=4:1 v/v), the final hydrogel is injectable through a syringe. This injectable property give a further advantage of 3D cell culture system by creating the possibility for in vivo study with the same platform system. Cells or molecular compounds which have been successfully tested in vitro 3D cell culture system can be injected into animals using the same system for in vivo study. In combine with existing/novel analysis technology, we hope our technology can shorten the R&D time of drug discovery, cell therapy and tissue engineering studies and benefit pharmaceutical and clinical industries.