VitroGel 3D simplifies the hydrogel 3D cell culture procedure from hours to minutes. The whole 3D cell culture process can be done in 20 min including a 10-15 min waiting time for hydrogel stabilization. The room temperature stable solution makes 3D cell culture easy to operate. Watch the video to see how much time you can save by using the VitroGel 3D system.
2D Cell Coating Culture with VitroGel 3D Hydrogel System
VitroGel 3D can be used to coat the substance that cells grow on. The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study. Just like 3D cell culture, the operation of 2D coating can be done by a simple mixing step in 20 minutes, including a 10-15 minute waiting time for hydrogel stabilization.
3D 2D Cell Harvesting with VitroGel 3D Hydrogel System
After 3D cell culture, cells can also be easily harvested from the hydrogel by using standard centrifuging methods. With this capability, downstream analysis can be done with cells cultured with VitroGel 3D system. This video shows how easily cells are harvested with the VitroGel 3D system.
By mixing VitroGel™ 3D and cell culture media in a proper ratio, the hydrogel becomes injectable for multiple applications. The hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times. The concentration of hydrogel solution can also be adjusted for different hydrogel strengths. Multiple applications can be performed with these flexibilities.