VitroGel 3D-RGD is a ready-to-use tunable hydrogel system modified with RGD cell adhesive peptide, promoting the cell attachment and cell-matrix interactions during the 3D cell culture. This RGD modified hydrogel system is good for adhesion cells or cells need good cell-matrix interactions.
- Xeno-free hydrogel modified with RGD peptide
- Good for adhesion cells or cells require good cell-matrix interactions
- Ready-to-use at room temperature
- Tunable hydrogel: dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations
- Neutral pH
- Compatible with VitroGel Cell Recovery Solution for easy cell harvesting
- Injectable hydrogel (Check user handbook for preparation details)
- Ships room temperature. Store at 2-8 °C
- Size: 2 mL and 10 mL
- Number of uses (10 mL): 2-6 of 24-well plate at 250 to 300 µL/well
Figure 1: 3D culture of PANC-1 cells at three different dilutions of VitroGel 3D-RGD.
PANC-1 cells were cultured in increasing dilutions of hydrogel solution at concentrations of 1:0, 1:1, and 1:3. The morphological differences were seen based on the concentration of the hydrogel. The 1:1 and 1:3 dilution ratios of hydrogel allowed for more rapid division and expansion of the single cells, developing larger 3D colonies compared to 1:0 dilution. The ActinGreen / NucBlue staining in the enlarged images (bottom) reveals increasing complexity and dispersion of the ECM generated by the cell-cell contact of PANC-1 cells when maintained in VitroGel 3D-RGD hydrogel.
Images were taken at Day 7 with 10X (top and middle) and 30X enlarged (bottom); corresponding antibody staining: NucBlue (nucleus) and ActinGreen (actin).
Figure 2. 2D and 3D cell culture of Human colon cancer cells (HCT 116) cells cultured on of VitroGel 3D-RGD hydrogel
On 2D surface of VitroGel 3D-RGD, cells spread out, adopting a morphology reminiscent of the 2D culture on standard treated culture plastic. When cultured in 3D hydrogel, the strength of the hydrogel (different dilution) can affect its mechanical properties, and thus cell viability and response within the hydrogel. The results show that the 1:1 hydrogel dilution allowed for big colonies which can mimic in vivo situation for drug screening (see HCT 116 application note for details).
A thick hydrogel coating plate has been prepared by mixing VitroGel 3D with PBS at 1:1 ratio. A 300 µL mixture has been added to a well of a 24-well plate and stabilization at room temperature for 20 min before adding cells on top of the hydrogel. Cell spheroids form on the top of hydrogel. Cells seeded at 2.5-10×105 cells/mL.
Figure 3. 3D culture of Beta TC3 and Ins-1 cells in VitroGel 3D-RGD hydrogel
Both Beta TC3 and Ins-1 cells can form a big spheroid structure in VitroGel 3D-RGD after 14 day. The cells were seeded at 5×105 cells/mL
Please check the examples of cells that have been successfully tested on VitroGel 3D-RGD: Click here
Download first-time user note: Click here
Download user handbook: Click here
View Video Protocols on 3D cell culturing and 2D coating method: Video Protocol Demonstration
View Video Protocol on Cell Recovery from the hydrogel: Video Protocol Demonstration
Download SDS: Click here
Download product brochure: Click here
For research use only. Not for use in diagnostic or therapeutic procedures.