figure 3 3D hPSC spheroids from LN2

Figure 3. 3D hPSC spheroids can be established directly from cells stored in liquid nitrogen (LN2)
A. hPSC spheroids formed directly from cells stored in liquid nitrogen grow continuously from day 1 to 6. B. Alkaline Phosphatase (AP) staining shows that these spheroids are healthy and that they retain their pluripotency indicated by high levels of AP expression.