Which VitroGel® hydrogel product should I choose?

Ready-To-Use Hydrogels #

For many users, the “Ready-To-Use” hydrogels are preferred for many applications. You need only to add your cells, cover the medium, and incubate. The hydrogel versions have been formulated and optimized for different cell types and applications.

• VitroGel® Hydrogel Matrix (Cat#: VHM01): This is the recommended hydrogel for many users, specifically for 3D cell culture and 2D coating. It is a formulation of multi-functional ligands and concentrations to support various cell types for different applications. It is a ready-to-use hydrogel; you only need to add your cells, cover the medium, and incubate.

High Concentration (Tunable) Hydrogels – “Building Blocks for Tissue Engineers” #

For more advanced users, the high-concentration hydrogels give scientists full control to manipulate the biophysical and biological properties of the cell culture environment.  All the high-concentration hydrogels come with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4,000 Pa. Customized hydrogels can reach more than 20,000 Pa. We have many types of hydrogel with different binding ligands, making our system a “Mix and Match” where you can control what is in the hydrogel for your 3D cell culture.

Create your own ECM.  Create over 100 possible combinations using the high-concentration hydrogels.

• VitroGel® 3D High Concentration Kit (Cat#: TWG001) is a pure, unmodified hydrogel that allows maximum flexibility to manipulate the 3D cell culture environment for different needs. The unmodified hydrogel matrix structure is suitable for cell spheroid formation, suspension cells, or cells requiring low cell-matrix interactions.
• VitroGel® RGD High Concentration Kit (Cat#: TWG003) is modified with a high concentration of RGD cell adhesive peptides, promoting cell attachment and cell-matrix interactions during the 3D cell culture. It can achieve high levels of integrin-binding activities to promote intercellular networks even after hydrogel dilution.
• VitroGel® COL High Concentration Kit (Cat#: TWG009) is modified with a collagen-mimetic (GFOGER) peptide that specifically binds the integrin α2β1. This promotes many bioactivities, such as osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo.
• VitroGel® MMP High Concentration Kit (Cat#: TWG010) is modified with Matrix Metalloproteinases (MMP) for a biodegradability matrix. We can customize our hydrogels with different functional ligands. Please contact support@thewellbio.com if you need a customized product.
• VitroGel® IKVAV High Concentration Kit (Cat#: TWG007) is modified with laminin-derived functional peptide, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth.
• VitroGel® YIGSR High Concentration Kit (Cat#: TWG008) is modified with a laminin-derived functional peptide that involves endothelial cell adhesion, cell proliferation, and motility/migration.

We can customize our hydrogels with different functional ligands. Please contact support@thewellbio.com if you need a customized product.

If you need help finding the right hydrogel product for your research project, please fill out this product help form: https://www.thewellbio.com/vitrogel-product-help/