The hydrogel cross-link initial when the hydrogel solution mixed with cell medium. The mixture will increase the viscosity immediately, which will ensure the cells are homogenously suspended in hydrogel matrix. If needed, customers can put the cell-VitroGel® mixture at low temperature (4°C or ice bucket) to accelerate the hydrogel formation rate.
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When the mixture of the cells and the VitroGel® is left to stabilize at room temperature before the injection, will the suspension cells sink to the bottom of the tube?
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