Author Archives: developer1

3D Printed Tumor Spheroids for Disease Modeling and Chemotherapeutic Drug Screening

Poster Presentation TheWell Bioscience Inc. Aragaw Gebeyehu1, Sunil Surapaneni1, John Huang2, Arindam Mondal1, Vivian Wang2, Nana-Fatima Haruna2, Peggy Arthur1, Shallu Kutlehria1, Nil Patel1, Mandip Sachdeva1 1College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL3230; 2TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract 3D bioprinting is intended for regenerative medicine and disease model development […]

Characterizing the Biophysical Properties of a Tunable Hydrogel System for 3D Cell Culture

Poster Presentation TheWell Bioscience Inc. Nana Fatima Haruna, Ziwen Vivian Wang, John Huang TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract The hydrogel-based 3D cell culture model more accurately depicts the microenvironment of cells in vivo than the traditional 2D cell culture system, which enables cells to grow, develop, operate, and produce feedback as if […]

3D Cell Culture of Human Colon Cancer Cells (HCT116) on VitroGel® System

Application Note TheWell Bioscience Inc. Introduction In vitro assays have become increasingly popular in drug discovery applications because of their high throughput functionality and cost-effectiveness in conducting optimization and preclinical validation. Three-dimensional (3D) culture assays have become especially popular because compared to traditional two-dimensional (2D) cell culture, 3D culture provides a more physiologically relevant environment […]

3D Cell Culture with VitroGel High Concentration Hydrogel System

VitroGel® High Concentration hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. The whole 3D cell culture process can be done in 20 min including a 10-15 min waiting time for hydrogel stabilization. The room temperature stable solution makes 3D cell culture easy to operate. Watch the video to see how much […]

Cell Recovery from 3D or 2D culture in VitroGel Hydrogel System

After 3D or 2D cell culture, cells can also be easily recovered from the hydrogel by using our VitroGel Organoid Recovery Solution and a quick centrifuge. With this capability, downstream analysis can be done with cells cultured with the VitroGel system. This video shows how easily cells are recovered from the VitroGel hydrogel system. There […]

2D Cell Coating Culture with VitroGel High Concentration Hydrogel System

VitroGel can be used to coat the substance that cells grow on. The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study. Just like 3D cell culture, the operation of 2D coating can be done by […]

Injectable Hydrogel: VitroGel 3D System

Injectable hydrogel Injectable Hydrogel: VitroGel 3D System By mixing VitroGel™ 3D and cell culture media in a proper ratio, the hydrogel becomes injectable for multiple applications. The hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times. The concentration of hydrogel solution can also be adjusted for different hydrogel […]