Author Archives: thewellbio

3D Spheroid Invasion Assay With the Xeno-free, Bio-Functional VitroGel® Hydrogel Matrix

Application Note Nana-Fatima Haruna, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Cell migration and invasion play important roles in various physiological processes such as gastrulation, embryonic morphogenesis, development of the nervous system, and immune cell trafficking. Cells need to be able to change, interact with their environment, and reach their proper spatial orientation […]

Glucose Metabolism in Obese Mice can be Improved by Implantation with Photoactivated Adipose Stem Cells

Institution: Southwest Medical University, Luzhou, Sichuan, China Team: Zhu L., Feng Z., Shu X., Gao Q., Wu J., Du Z., Li R., Wang L., Chen N., Li Y., Luo M., and Wu J. Application: In vivo implantation of adipose stem cells to ameliorate glucose intolerance in obese mice Disease Model: Type 2 Diabetes Hydrogel: VitroGel® […]

Establishing the First Co-Culture Model of Human Gastric Organoids and Dendritic Cells Using the Xeno-free VitroGel ORGANOID Hydrogel

Institutions: Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, United States Team: Michelle D. Chergne, Barkan Sidar, T. Andrew Sebrell, Humberto S. Sanchez, Kody Heaton, Francis J. Kassama, Mandi M. Roe, Andrew B. Gentry, Connie B. Chang, Seth T. Walk, Mark Jutila, James N. Wilking, and Diane Bimczok Application: A Synthetic Hydrogel, […]

New hPSCs Expansion Method – Direct from Liquid Nitrogen to 3D Culture Using a Xeno-free Hydrogel – VitroGel® STEM

Culturing and maintaining human pluripotent stem cells (hPSCs) directly from cryopreservation using the xeno-free, hydrogel-based VitroGel® STEM Application Note TheWell Bioscience, North Brunswick, NJ 08902 Introduction Due to the advent of stem cell technologies, there has been an unprecedented rise in the use of human pluripotent stem cells (hPSCs) in biomedical research. Because of its […]

Identification of a Novel Antitumor Small-Molecule Known as MKI-1 via Activation of PP2A in Breast Cancer

Institutions:Korea Institute of Radiological and Medical Sciences, Seoul, South Korea; Radiological and Medico-Oncological Sciences, University of Science and Technology, Daejeon, South Korea; Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, South Korea; New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, South Korea; Center for Medicinal Chemistry, Korea Research Institute of Chemical Technology, Daejeon, South Korea […]

miR-340 Restoration Promotes Macrophage Phagocytosis and Antitumor Immunity via Regulation of CD47 Expression

Institutions: Tianjin Medical University, Tianjin, China; Guangdong Pharmaceutical University, Guangzhou, China; University of Texas Southwestern Medical Center, Dallas, Texas, USA. Team: Xi q., Zhang J., Yang G., Zhang L., Chen Y., Wang C., Zhang Z., Guo X., Zhao J., Xue Z., Li Y., Zhang Q., Da Y., Liu L., Yao Z., and Zhang R. Application: […]

KLF7: Discovery of a Novel Therapeutic Target and Biomarker for High-Grade Serous Ovarian Cancer

Institution: Department of Life Sciences and Public Health, Catholic University of the Sacred Heart, Milan, Italy Team:De Donato M., Babini G., Mozzetti S., Buttarelli M., Ciucci A., Arduini G., De Rosa M. C., Scambia G., Gallo D. Application:Discovery of KLF7, a novel biomarker and therapeutic target for high-grade serous ovarian cancer Disease Model:Ovarian Cancer Hydrogel:VitroGel® […]

Mesenchymal Stromal Cell-Derived CXCL12 Affects Tumor Growth by Macrophage M1 to M2 Polarization

Institution: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Team:Babazadeh S., Nassiri S. M., Siavashi V., Sahlabadi M., Hajinasrollah M., and Zamani-Ahmadmahmudi Z. Application:Identification of a new role for MSC-derived CXCL12 in M2 phenotypic switching of macrophages, affecting tumorigenesis in breast cancer cells Disease Model:Breast Cancer Hydrogel:VitroGel® RGD Studies over […]

New Findings Elucidate BMP4 and Decellularized Pulp Extracellular Matrix Synergy to Accelerate Tissue Formation by Stem Cells

Institution: Stomatological Hospital of Chongqing Medical University, Chongqing Medical University. People’s Republic of China Team: Tan Q., Cao Y., Zheng X., Peng M., Huang E., and Wang J. Application: Identification of a novel synergistic relationship between BMP4 and dECM to accelerate pulp-like tissue formation by DPSCs in vitro Disease Model: Dental pulp stromal cells Hydrogel: […]

Advanced Skin Cell Models Using the VitroGel® System: 2D Coating and 3D Cell Culture with Human Dermal Fibroblasts

Application Note Bhavya Shah, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Despite recent progression, the need for reliable, repeatable in vitro three-dimensional (3D) tissue culture human skin models exist to further understand the mechanisms behind various pathologies related to the skin and/or dermal fibroblasts1-3. Such 3D models are increasingly important as two-dimensional (2D) […]