Author Archives: thewellbio

MSC (Mesenchymal Stem Cell) 3D Culture Scale-Up | Bead-Droplet Protocol

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). The Bead-Droplet method can be used to make hydrogel cell beads for MSC scale-up and long-term culture. This novel culture method generates high-quality MSCs and eliminates the usage of microcarriers for MSC scale-up.

3D Invasion of Glioblastoma Cells in VitroGel® Hydrogel System

Application Note Nana-Fatima Haruna, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Glioblastoma is the most common primary tumor in the central nervous system. With an incidence of 3–5 out of 100,000 persons in Western countries, glioblastoma is one of the most deadly forms of malignant tumors, which has a median survival time of […]

Using the VitroGel® Hydrogel System as a Versatile Platform for Invasion Assay

Application Note Nana-Fatima Haruna, Atsuo Ochi, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Invasion studies have been crucial in identifying key intracellular signaling pathways and membrane-bound and soluble enzymes that influence cellular movement through a three dimensional (3D) extracellular matrix (ECM)1,2. Such information has been used to investigate mechanisms governing the infiltration of […]

Targeting Gangliosides for Melanoma Brain Metastasis Treatment

In vitro three-dimensional analysis elucidates the role of cell surface GD3 ganglioside and ST8SIA1 in melanoma brain metastasis. Institution: John Wayne Cancer Institute (JWCI), Fudan University, Providence St. Joseph Health Center, Tel-Aviv University, The University of Texas MD Anderson Cancer Center, Oregon Health and Science University (OHSU) Knight Cancer Institute Portland Team: Romela Irene Ramos, […]

Penetrating Cancer Stem Cells For Lung Cancer Treatment

Scientists from Florida A&M University have discovered the ability of Tel and DXTPL combination system to increase the power of DXTPL to penetrate cancer stem cells and decrease the chances of cancer relapse and metastasis. Institution: Florida A&M University Team: Peggy Arthur, Nilkumar Patel, Sunil Kumar Surapaneni, Arindam Mondal, Aragaw Gebeyehu, Arvind Bagde, Shallu Kutlehria, […]

Silent Partner: The MYC-related pseudogene HMGA1P6 drives malignancy in ovarian cancer

In vitro three-dimensional culture helps to demonstrate that several pseudogenes, including HMGA1P6, are involved in metastasis of ovarian cancer, introducing another unconventional target for cancer therapeutics. Institution: Shandong University Team: Xiaoxue Tian, Jianping Song, Xiyu Zhang, Mingyao Yan, Shourong Wang, Yuqiong Wang, Limei Xu, Ling Zhao, Jian-jun Wei, Changshun Shao, Beihua Kong *, and Zhaojian […]

The injectable VitroGel provides a viable in vivo scaffold for novel maternal-fetal adipose stem cell transplant studies

Maternally derived adipose stem cells have emerged as a possible solution to the common immunological rejection observed in fetal repair surgeries. Institution: Showa University School of Medicine Team: Akihiro Kawashima*, Rika Yasuhara, Ryosuke Akino, Kenji Mishima, Michiko Nasu, Akihiko Sekizawa Application: In vivo utilization of VitroGel RGD to study neuronal differentiation capacity Disease model: Neurometabolic […]