Cell invasion assay culture process in 30 minutes. Work confidently at room temperature. No ice bucket here. Traditional in vitro cell invasion assays have relied on animal-based extracellular matrices (ECM), which come with challenges like undefined components, batch-to-batch variability, and cumbersome temperature-sensitive protocols, thus leading to inconsistent and inaccurate studies. Watch how simple and […]
Category Archives: Video Protocols – 3D cell culture
Recovering organoids from an animal-based ECM is challenging. Sometimes, the process can take hours using either harsh chemical solutions, strong enzymes, or temperature adjustment, which can damage cells and reduce yield. VitroGel® Organoid Recovery Solution is a non-enzymatic cell harvesting solution for quick and efficient recovery of organoids/cells from VitroGel hydrogels or an animal-based […]
VitroGel xeno-free hydrogels are excellent for injection and a superior alternative to the animal-based extracellular matrix or plant-based hydrogel for xenograft, targeted delivery, control release, and cell therapy for tissue engineering. Simply mix hydrogel solution with cells/compounds at room temperature, the hydrogel is ready for injection in 5-15 minutes. The system is biocompatible without showing […]
VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells. Easily harvest HEK293 cells in less than 15 minutes with our VitroGel Cell Recovery Solution.
VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells. Simple 5 minute protocol for 3D static suspension culture for HEK293 cells.
* The video is referencing VitroGel Cell Recovery Solution. Use VitroGel Organoid Recovery Solution in its place. Recovering organoids from a 3D hydrogel system is challenging. Sometimes, the process can take hours using either harsh chemical solutions, strong enzymes, or temperature adjustment, which can damage cells and reduce yield. With VitroGel® ORGANOID, recovering organoids […]
VitroGel® ORGANOID are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs). Organoids are further easily harvested with our enzyme-free cell recovery solution, VitroGel Cell Recovery Solution.
VitroGel® ready-to-use hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. Just mix with your cells. There is no crosslinking agent or the need to adjust the hydrogel concentration. The whole 2D coating protocol can be done in 20 minutes (including a 10-15 min waiting time for hydrogel stabilization). The room temperature […]
Easy 5-minute protocol for harvesting hPSC spheroids cultured in VitroGel STEM.
5-minute protocol for suspension culture of hPSC using VitroGel STEM.
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