Category Archives: Video Protocols – 3D cell culture

VitroGel®-Based Cell Invasion Assay Kits – 30 minute protocol

Cell invasion assay culture process in 30 minutes. Work confidently at room temperature. No ice bucket here. Traditional in vitro cell invasion assays have relied on animal-based extracellular matrices (ECM), which come with challenges like undefined components, batch-to-batch variability, and cumbersome temperature-sensitive protocols, thus leading to inconsistent and inaccurate studies.   Watch how simple and […]

Harvest Organoids/Cells From Animal-Based ECM With VitroGel® Organoid Recovery Solution

Recovering organoids from an animal-based ECM is challenging. Sometimes, the process can take hours using either harsh chemical solutions, strong enzymes, or temperature adjustment, which can damage cells and reduce yield.​   VitroGel® Organoid Recovery Solution is a non-enzymatic cell harvesting solution for quick and efficient recovery of organoids/cells from VitroGel hydrogels or an animal-based […]

VitroGel Hydrogel Injection

VitroGel xeno-free hydrogels are excellent for injection and a superior alternative to the animal-based extracellular matrix or plant-based hydrogel for xenograft, targeted delivery, control release, and cell therapy for tissue engineering. Simply mix hydrogel solution with cells/compounds at room temperature, the hydrogel is ready for injection in 5-15 minutes. The system is biocompatible without showing […]

3D Organoid Harvesting

* The video is referencing VitroGel Cell Recovery Solution.  Use VitroGel Organoid Recovery Solution in its place. Recovering organoids from a 3D hydrogel system is challenging. Sometimes, the process can take hours using either harsh chemical solutions, strong enzymes, or temperature adjustment, which can damage cells and reduce yield.​   ​With VitroGel® ORGANOID, recovering organoids […]

2D Coating Culture with VitroGel “Ready-To-Use” Hydrogels

VitroGel® ready-to-use hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. Just mix with your cells. There is no crosslinking agent or the need to adjust the hydrogel concentration. The whole 2D coating protocol can be done in 20 minutes (including a 10-15 min waiting time for hydrogel stabilization). The room temperature […]