We are here to help you enjoy your scientific exploration. Search our frequently asked questions. If your question still has not been answered, email us at support@thewellbio.com.
Popular Search
stiffnessorganoidcyto3dgelationstiff
Hydrogel / Cell Preparation
- How do I prepare the cell suspension to mix with the hydrogel? Should I add serum?
- How do I adjust the hydrogel formation time?
- How fast should I transfer the sample from the mixing tube to the culture plate?
- When the mixture of the cells and the VitroGel® is left to stabilize at room temperature before the injection, will the suspension cells sink to the bottom of the tube?
- Can I use a serum-free medium with VitroGel® ?
- Will the use of 100% FBS reduce the viscosity of the tunable gel when being diluted?
- Can I add extracellular matrix proteins or other molecular compounds into VitroGel®?
- Are there visual or other methods can be used to verify the status of hydrogel formation?
- Can we use multichannel pipette for hydrogel mixture?
- What can I do to prevent bubbles from forming when mixing the VitroGel® solution with the cell culture medium?
- What type of culture plate should I choose when using VitroGel®?
- Why does the hydrogel sticks loosely to the tissue culture plate?
- How to improve the hydrogel attachment on the cell culture plate/glass bottom well plates?
- How does the VitroPrime™ Spread-Attach Plate help for viscous hydrogel (such as high concentration VitroGel®) that has slow spreading on the surface of culture plate?
- Will adjusting the temperature induce the hydrogel formation of VitroGel®?
- How to achieve different stiffness with the same functional RGD-ligands by combining the VitroGel® RGD with VitroGel® 3D in various mixing and dilution ratios?
- Is it possible to dilute VitroGel® hydrogels with cell culture medium instead of the dilution solution?
- What is the difference between 3D culture and 2D hydrogel coating culture?
- How can I prevent some cells from being attached to the bottom resembling 2D coating when using VitroGel® for 3D cell culture?
- Can cells grown in the VitroGel® be sub-cultured?
- How often does the cover media need to be changed for 3D culture?
- Shall I change the full amount of the cover medium or just partially?
- Can we use the same cell seeding number of Matrigel for VitroGel® system?
- What cell seeding density should I use?
- How stable of VitroGel® Microbead in cell culture medium?
- Can I dissociate hydrogel directly inside of well plate without removing it out for high-throughput application?
- Can I place the gel mixture in an incubator instead of leaving it at room temperature for gel stabilization?
- What is the optimal ratio between cell suspension and gel for the mix?
- What is the optimal incubation time?
- Can I pre-coat the culture plate to increase hydrogel attachment?
