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Viability
- Is there a threshold or limitation for the cell confluency for the Cyto3D® Live-Dead Assay Kit?
- Is a final washing step required when using Cyto3D® Live-Dead Assay Kit with VitroGel®?
- Can Cyto3D® Live-Dead Assay Kit be used in other matrices such as Matrigel? Can it be used for other applications other than hydrogel based 3D cell culture?
- How long can fluorescence of Cyto3D® Live-Dead Assay Kit be maintained when 2D or 3D cells are cultured with this product? Is it possible to observe the fluorescence for days without any cytotoxic effect?
- What is the best technique to score the viability in intact 3D spheres? Can Cyto3D® Live-Dead Assay Kit be used in fixed cells too or it has to be live spheroids only?
