Yes, VitroGel® can be injected before or after soft hydrogel formation for in vivo study.
Before the hydrogel formation, the VitroGel® solution can be injected directly into the animal. It will become a hydrogel when it encounters the ionic compounds of the physiological environment.
After soft hydrogel formation, the VitroGel® hydrogel has an advanced injectable property. Mixing the hydrogel solution and cell culture media/PBS at a proper ratio makes the final hydrogel injectable for in vivo studies. Using this method, cells or other chemical compounds can be mixed in the hydrogel before injection.