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Do you have any tips on how to use VitroGel® ORGANOID instead of animal-based ECM for organoid culture?
- Add 3X of your medium supplement (e.g., R-Spondin, Noggin, etc) to the cell suspension before mixing with hydrogel at a 2:1 ratio. This way, you can ensure the hydrogel supplement is at least 1X. (you can prepare a 5X supplement in cell suspension if you like to promote the initial cell survival and growth)
- Increase the initial survival rate of organoids by seeding as a small colony instead of single cells and add ROCK inhibitor (such as Thiazovivin)
- If you want to mix VitroGel® with BME, please add 10-20% BME to the cell suspension before mixing with VitroGel®.
- For crypt, you can dilute VitroGel® with DI water at a 1:1 ratio before mixing with cell suspension; we found that crypts have lower gel strength.
