For high-concentration VitroGel®, can I prepare a pre-diluted solution by mixing VitroGel® with VitroGel® Dilution Solution and storing it in the fridge?

In most cases, we do not recommend preparing pre-diluted VitroGel®. Once the VitroGel® solution is mixed with the dilution solution, the hydrogel will begin to crosslink slowly. Although this initial gelation keeps the gel injectable, once crosslinking starts, the functional ligands in the hydrogel will gradually be neutralized by the salts in the medium over time.

Ideally, we recommend preparing the hydrogel fresh for each use. The non-diluted VitroGel® solution can be stored at 4°C for several months. There is no need to pre-dilute or pre-mix the hydrogel before use.