What is the optimal ratio between cell suspension and gel for the mix?

The mixing ratio for the Ready-To-Use VitroGel® Hydrogel system (blue cap) is 2:1. For our High-concentration VitroGel® system (red cap), it is 4:1. The mixing ratio is also tunable depending on the cell culture medium. For example, if the cell medium has a low ionic molecule concentration, such as RPMI, we recommend a 1:1 mixing ratio for ready-to-use hydrogel.

2D Coating, 3D Culture, Coating, Gelation, Hydrogel

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General Hydrogel Questions

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