2D Cell Coating Culture with VitroGel High Concentration Hydrogel System

VitroGel can be used to coat the substance that cells grow on. The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study. Just like 3D cell culture, the operation of 2D coating can be done by a simple mixing step in 20 minutes, including a 10-15 minute waiting time for hydrogel stabilization.

There are two methods to do the 2D coating:

• Method 1:  Standard Method
• Method 2:  Method to prepare a thin or thick gel coating for later use. Overnight incubation is required.

2D Coating Protocol:  Method 1

Method 1:  Standard Method

2D Coating Protocol:  Method 2

Method 2:  Method to prepare a thin or thick gel coating for later use. Overnight incubation is required.