Cell Recovery from 3D or 2D culture in VitroGel Hydrogel System

After 3D or 2D cell culture, cells can also be easily recovered from the hydrogel by using our VitroGel Organoid Recovery Solution and a quick centrifuge. With this capability, downstream analysis can be done with cells cultured with the VitroGel system. This video shows how easily cells are recovered from the VitroGel hydrogel system.

There are two methods to recover cells from the hydrogel:

• Method 1:  Uses a cut pipette tip to break and remove the hydrogel
• Method 2:  Uses a spatula to remove the hydrogel

[This page has been updated to reflect our new recovery solution:  VitroGel Organoid Recovery Solution.  The videos may refer to the VitroGel Cell Recovery Solution, the protocol is the same for the VitroGel Organoid Recovery Solution.] 

Cell Recovery Protocol: Method 1

Method 1: Uses a cut pipette tip to break and remove the hydrogel for cell recovery.

Cell Recovery Protocol: Method 2

Method 2: Uses a spatula to remove the hydrogel for cell recovery.