VitroGel™ 3D is used for 3D cell
culture, 2D coating and more…

flexible and versatile on many applications

Multiple applications of VitroGel 3D

3D cell culture

VitroGel 3D can be used for 3D cell culture by simply mixing with cells. The hydrogel matrix supports cells to grow as 3D colonies over time. Drugs or other chemical compounds can be added to penetrate into hydrogel system for screening.

2D coating

VitroGel 3D can be used to coat the substance that cells grow on. The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study.

and More…

By mixing the hydrogel solution and cell suspension (or other ionic solution) in a proper ratio (recommend 4:1 v/v), an injectable hydrogel can be formed and used for xenograft, drug delivery, bio-printing, microarray, etc.
Additionally, the hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times. The concentration of hydrogel solution can also be adjusted for different hydrogel strengths. Multiple applications can be done with these flexibilities.

For customized products or special applications, please contact us!

Watch demonstration videos or download our user guidelines for more protocols

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Download guidelines

VitroGel 3D has been successfully used on different projects with many cell lines such as human and mouse pancreatic beta cells, lymphocytes, Hela cells, hFS iPSCs, breast cancer cell lines and much more. The results show that cells can successfully grow in both 3D culture and 2D coating. Media can easily penetrate the hydrogel matrix and provide nutrition for long-term cell culture. The growth of cells can be observed easily under microscopy. Some case studies are listed below:

Case 1: 3D cell culture of Beta TC3 cells for 14 days

Seeding number: 5×105 cells/mL; Product: VitroGel 3D-RGD; all images were taken under the same scale.

Case 2: 3D cell culture of Ins-1 cells for 14 days

Seeding number: 5×105 cells/mL; Product: VitroGel 3D-RGD; all images were taken under the same scale.

See More Case Studies

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