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Do I need to keep the VitroGel®-Cell mixture in an ice bucket?
No. Unlike Matrigel, VitroGel® is ready-to-use and can mix with a cell suspension at room temperature. If you are wondering how gelation works, please visit: How Gelation Works With VitroGel® Hydrogels.
After the hydrogel and cell suspension are mixed and transferred to a syringe, you can put the mixture on ice, or 4°C, for 5-10 minutes to accelerate the injectable hydrogel formation and ensure a homogenous cell suspension. After that, the hydrogel can be kept at room temperature for long-term injection.
If you don’t want to use cold temperatures for any part of the protocol, you can leave the hydrogel-cell mixture at room temperature for 15 minutes for gel stabilization before injection. VitroGel® has a unique shear-thinning and rapid recovery property, which can maintain a long-term injectable status (hours) and excellent cell retention after injection without the needle clogging issue.
For more details, please refer to: Unique injectable properties of VitroGel®
