VitroGel® NEURON
Ready-to-use, xeno-free hydrogel system for neural stem cell and neuron cultures.
VitroGel® NEURON for 3D Neuron Culture
Xeno-free and biofunctional
100% synthetic. Animal & human origin-free hydrogel. No growth factors or proteins in the gel solution.
Room temperature operation
Stable at room temperature. Easy handling. Gelation is not temperature dependent. Learn how gelation works >
Supports 3D and 2D neuronal cell culture
Suitable for 3D and 2D culture of immortalized neuronal neuroblasts, iPSC-derived NSCs, neuron, and culturing of neural stem cells (NSCs) as single cells or neurospheres.
Injectable hydrogel for cell therapy
VitroGel® NEURON’s unique injectable properties make it an injectable carrier for neuron-based cell therapy, spinal cord injury repair, and other regenerative medicine applications.
VitroGel® NEURON is the first-of-its-kind synthetic, xeno-free hydrogel specifically engineered to support both 2D and 3D neuronal culture and in vivo delivery. This groundbreaking platform eliminates the variability of animal-derived ECMs, such as Matrigel®, and provides a defined, reproducible, and scalable environment for neuronal growth, differentiation, and network formation.
With its ready-to-use, room-temperature-operation formulation, VitroGel® NEURON streamlines workflows, is transparent, and imaging-friendly, enabling real-time visualization of neural development. The system is optimized for primary cells, cell lines, iPSC-derived neural stem cells, and neuron maturation in both 2D and 3D formats, making it a powerful tool for translational neuroscience research.
By combining a precision bio-functional microenvironment and injectable-friendly features, VitroGel® NEURON bridges the gap between basic research and future biomedical applications.
Superior for 2D Thin Coating over Animal-based ECM
200X Dilution
4X more usage than animal-based ECM
Long-term Culture
Over 4 weeks of maintenance and
post-differentiation culture.
Easy and Fast Operation
No need to remove the coating solution,
just seed cells directly.
3D Neuron Culture Made Easy with VitroGel® NEURON
3D Neuron Differentiation
Supports both single cells and neuron spheroids.
Excellent Cell-Cell Communication
Enhanced cell extension and cell-matrix interactions.
Imaging-Friendly
Transparent and direct staining within the hydrogel for high-quality image data.
Specifications
| Formulation | Xeno-free, biofunctional hydrogel |
| Use | 3D and 2D neuron and neural stem cell culture |
| Operation | Ready-to-use at room temperature |
| Injection | Injectable hydrogel for in vivo studies and lab automation |
| pH | Neutral |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Sizes | 10 mL and 2 mL |
Recommended Products
VitroPrime™ Spread-Attach Plate, combined with VitroGel® NEURON, supports exceptional 2D and 3D neuron growth. No PDL-coated plates required.
VitroPrime™ Spread-Attach Plate
RocketCell™ NSC Medium and Kit
Data and References
2D THIN COATING METHOD
2D: CASE 1
2D NSC Maintenance (1:200 coating on VitroPrime™ Spread-Attach Plate)
Figure 1. VitroGel® NEURON 2D Thin Coating for Growth of iPSC-Derived NSCs and Confluence Analysis on VitroPrime™ Spread-Attach Plates.
A. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with either VitroGel® NEURON (1:200) or Geltrex®, and cultured for 6 days. Images were captured using the Incucyte® S3 system. B. NSC growth was quantified by percent confluence over time, with images collected every 8 hours and analyzed relative to the initial timepoint (T = 0 + 4 hrs).
2D: CASE 2
2D B35 Neuronal Neuroblast Differentiation (2D thin coating method)
Figure 2. 2D thin coating method using VitroGel® NEURON hydrogel promotes B35 neuronal neuroblast differentiation.
Immunofluorescence staining of neuronal cultures was performed to evaluate the presence of the neuron-associated marker beta-III-tubulin on day 7 post-differentiation induction. The cultures were visualized with the Keyence BZ-X system.
2D: CASE 3
2D NSC Dopaminergic Differentiation
A
Figure 3. 2D thin coating with VitroGel® NEURON hydrogel supports the development of Dopaminergic Neuron Progenitors (DNPs)
A. Video shows robust survival of DNP colonies, and elaboration of neurites; B & C. IncuCyte Neurotrack analysis software was used to quantify the expansion of the area of DNP colonies, and their enhanced complexity of neurite elongation.
B
C
2D HYDROGEL COVERING “BLANKET METHOD”
2D BLANKET: CASE 4
2D B35 Neuronal Neuroblast Differentiation (2D “blanket” method)
Figure 4: 2D “Blanket” method using VitroGel® NEURON hydrogel sustains in vitro neuronal differentiation. Immunofluorescence staining of neuronal cultures was performed to evaluate the presence of the neuron-associated marker beta-III-tubulin on days 7, 14, 21, and 30 post-differentiation induction. A. Light microscopy image showing neuronal cultures. B. Green-fluorescent image illustrating the presence of beta-III-tubulin. C. The nuclei were observed using DAPI (blue) staining. D. Combination of B and C images. The cultures were visualized using the Molecular Devices Image Xpress Nano system at a 20X magnification.
3D CELL CULTURE
3D: CASE 1
Long-term 3D neuronal differentiation (B35 cells))
Figure 5. VitroGel® NEURON sustains long-term 3D neuronal differentiation.
Bright field microscopy images depicting 3D B35 neuronal neuroblast differentiation in VItroGel® NEURON on days 7, 14, and 21. The images were obtained with the Keyence BZ-X microscope.
3D: CASE 2
3D NSC culture with VitroGel® NEURON as single cell encapsulation
Figure 6. 3D encapsulation culture of NSC cells as a single cell suspension in VitroGel® NEURON
3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC Xeno-Free Medium and mixing with VitroGel® NEURON for 6 days. A. Light microscopy images of NSC cultures demonstrating that VitroGel® NEURON Hydrogel and RocketCell™ NSC Xeno-Free Media Support neurite outgrowth. B. Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The image was obtained with the Keyence BZ-X microscope at a 20X magnification.
3D: CASE 3
3D Development of Dopaminergic Neurons from NSC spheroids
A
B
Figure 7. VitroGel® NEURON hydrogel supports the 3D development of dopaminergic neurons.
iPSCs were 3D cultured in RocketCell™-IPSC Media with VitroGel® STEM for 6 passages and were differentiated into Floor Plate Neuro-Epithelial
Spheroids.The spheroids were recovered from the hydrogel using the VitroGel® Organoid Recovery Solution and embedded in VitroGel® NEURON.
A. Brightfield images of spheroid on day 0 (A, left) that were differentiated for 16 days (A, right). B. The samples were fixed and stained with beta-III-tubulin antibody and DAPI, depicting that the majority of the spheroids are neurons with the ability to form axonal connections. The cultures were visualized with the Keyence BZ-X microscope at 4X and 10X magnifications.
Enhance your neuron cell culture with these products:
New
Cell Culture Plates
Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.
CytoGrow™ - Recombinant Proteins
Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies.
Cell Harvesting Solution
Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Improved formulation over VitroGel® Cell Recovery Solution.
Biomarkers
$238.00
Versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
| Size | 2 mL, 10 mL |
|---|
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