The bubble issue is related to increased viscosity after mixing the gel solution with the cell medium. Here are some suggestions that can help to reduce the formation of bubbles:
- Warm up the VitroGel® solution to 37°C to reduce the viscosity of the gel.
- Gently mix the VitroGel® solution with the cell medium. Then, pipette slowly without introducing bubbles.
- Quickly spin the mixing tube to get rid of bubbles.