Category Archives: Video Protocols – 3D cell culture

3D Cell Culture with VitroGel Ready-To-Use Hydrogels

VitroGel® ready-to-use hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. Just mix with your cells.  There is no crosslinking agent or the need to adjust the hydrogel concentration. The whole 3D cell culture process can be done in 20 min including a 10-15 min waiting time for hydrogel stabilization. The room […]

MSC (Mesenchymal Stem Cell) 3D Culture Scale-Up | Bead-Droplet Protocol

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). The Bead-Droplet method can be used to make hydrogel cell beads for MSC scale-up and long-term culture. This novel culture method generates high-quality MSCs and eliminates the usage of microcarriers for MSC scale-up.

3D Cell Culture with VitroGel High Concentration Hydrogel System

VitroGel® High Concentration hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. The whole 3D cell culture process can be done in 20 min including a 10-15 min waiting time for hydrogel stabilization. The room temperature stable solution makes 3D cell culture easy to operate. Watch the video to see how much […]

Cell Recovery from 3D or 2D culture in VitroGel Hydrogel System

After 3D or 2D cell culture, cells can also be easily recovered from the hydrogel by using our VitroGel Organoid Recovery Solution and a quick centrifuge. With this capability, downstream analysis can be done with cells cultured with the VitroGel system. This video shows how easily cells are recovered from the VitroGel hydrogel system. There […]

2D Cell Coating Culture with VitroGel High Concentration Hydrogel System

VitroGel can be used to coat the substance that cells grow on. The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study. Just like 3D cell culture, the operation of 2D coating can be done by […]

Injectable Hydrogel: VitroGel 3D System

Injectable hydrogel Injectable Hydrogel: VitroGel 3D System By mixing VitroGel™ 3D and cell culture media in a proper ratio, the hydrogel becomes injectable for multiple applications. The hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times. The concentration of hydrogel solution can also be adjusted for different hydrogel […]