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Organoids
- Can I form a dome shape culture?
- Should I use treated or non-treated culture for dome methods?
- Which VitroGel® ORGANOID hydrogel should I choose for my organoid type?
- What are the differences between the 4 VitroGel® ORGANOID versions?
- What are the different mechanical strengths between VitroGel® ORGANOID versions?
- If I can’t screen all four organoid formulations, which is the one that acts as a good start?
- After screening the four formulations of the organoids, what factors can be used to determine the optimal formation for further experimentation?
- Do you have any tips on how to use VitroGel® ORGANOID instead of animal-based ECM for organoid culture?
- Can organoids be passed after growing them in VitroGel®?
- How often is it recommended to harvest the organoids grown using the VitroGel® ORGANOID?
- When transferring organoids from Matrigel to VitroGel®, do you recommended gradually decreasing the % of Matrigel in VitroGel®?
- Can VitroGel® Organoid Recovery Solution replace trypsin for cell dissociation?
- Can VitroGel® Organoid Recovery Solution use to dissolve GelMA, collagen, PEG based hydrogel or alginate?
- Any additional tip for recovering organoid from Geltrex by using VitroGel® Organoid Recovery Solution (MS04)?
- What are the differences in the harvesting protocol when recovering intact organoids versus organoid fragment (dissociated organoid)? Does incubation time impact organoid integrity, or is it primarily influenced by mechanical factors such as the type of pipette used?