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Can I use VitroGel® for Spheroid Invasion Assay? What is the protocol?
Yes, VitroGel® is excellent for Spheroid Invasion Assay application.
Here are some steps that could be followed:
- Add cells to the well plate first,
- Centrifuge to make sure cell aggregate at the bottom of the plate,
- Try to remove the medium (or in step 1 add as little medium as possible),
- Prepare the hydrogel with cell medium (without cells at a 2:1 mixing ratio),
- Add the gel to each well (on top of the cell spheroid),
- Centrifuge the plate again to make sure the gel and cell spheroid mix well,
- Wait for 10-15 min at RT, then add the cover medium.
Please check the application note of VitroGel® spheroid invasion assay
For more information, visit the VitroGel® invasion application page
Related products: VitroGel® Invasion Assay, VitroGel® Hydrogel Matrix
