Do I have to add serum, growth factors, or supplements to the cell suspension before mixing with VitroGel®?

No, adding supplements to the hydrogel matrix is not required. For most xenograft applications, preparing cells in a PBS solution works exceptionally well with VitroGel®.

VitroGel® is also animal-origin-free, which allows researchers to manipulate the supplement in a gel-cell mixture if needed.