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How can I decide the mixing ratio between ready-to-use hydrogel solution and cell suspension while preparing samples for xenograft application?

If the cell suspension is prepared with a culture medium such as DMEM or DMEM/F12, we recommend keeping a 2:1 mixing ratio. On the other hand, if the suspension is prepared in PBS or a culture medium with a low ionic concentration, such as RPMI, a 1:1 mixing ratio can be used.