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Is it possible to dilute VitroGel® hydrogels with cell culture medium instead of the dilution solution?
For the High Concentration hydrogels, it is not recommended. The concentration of the ionic molecule in medium would be much higher than the dilution solution, so diluting VitroGel® with cell culture medium may cause the gel to form too quickly and chunky. We recommend using VitroGel® Dilution Solution to adjust the highly concentrated VitroGel®.
Alternatively, VitroGel® Hydrogel Matrix (Cat# VHM01) is a better option for scientists who do not want to worry about dilution and only want to directly mix the hydrogel with cell suspension.
