For the High Concentration hydrogels, it is not recommended. The concentration of the ionic molecule in medium would be much higher than the dilution solution, so diluting VitroGel® with cell culture medium may cause the gel to form too quickly and chunky. We recommend using VitroGel® Dilution Solution to adjust the highly concentrated VitroGel®.
Alternatively, VitroGel® Hydrogel Matrix (Cat# VHM01) is a better option for scientists who do not want to worry about dilution and only want to directly mix the hydrogel with cell suspension.