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What cell seeding density should I use?
The final cell concentration can be optimized based on different cell types. We recommend preparing cell suspension at the following concentration:
- 3D cell cultures:
- For Ready-to-use hydrogels: 1-2 x 106 cells/mL (the cell suspension needs to mix with VitroGel® solution at the 2:1 ratio (VitroGel® solution: Cell suspension at 2:1 v/v), which makes the final cell concentration in the hydrogel 0.5-1 x 105 cells/mL)
- For the High-Concentration hydrogels: 0.5-2 x 106 cells/mL (the cell suspension needs to mix with VitroGel® solution at the 4:1 ratio (VitroGel® solution: Cell suspension at 4:1 v/v), which makes the final cell concentration in the hydrogel 1-4 x 105 cells/mL)
- 2D hydrogel coating: 1-5 x 105 cells/mL
