What is the best technique to score the viability in intact 3D spheres? Can Cyto3D® Live-Dead Assay Kit be used in fixed cells too or it has to be live spheroids only?

Cyto3D® Live-Dead Assay Kit is designed for cell viability testing. Software (ImageJ/Fiji) can quantify the GFP and TxRed intensity to quantify viability in culture, giving the percent of viability. The automatic cell counter or plate reader can also be used with Cyto3D® Live-Dead Assay Kit for cell suspension or 2D cell culture.

Imaging, Spheroid, Viability

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